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For over three decades, the wax layer has been considered to be synthesized by the follicle cells that surround the oocytes, exclusively based on electron microscopy observation of lipid endosomes within the follicle cells during oogenesis and their eventual deposition onto the vitelline membrane of the egg [46, 47]. Most of the hydrocarbons we detect in the wax layer are so far only known to be synthesized in the oes [39]; for example, the biosynthesis of dienes like 7,11-HD and 7,11-ND (female-specific pheromones) requires the enzymatic action of a specific desaturase desatF (Fad2) in the oes [48]. However, transcriptional data (microarray and RNA-sequencing [RNA-seq] data from FlyAtlas and FlyBase, respectively) show that this gene is not expressed in the ovary. Thus, further empirical investigations are necessary to clarify the role of follicle cells in the synthesis of the wax layer. 59ce067264
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